study of crocin & radiotherapy-induced cytotoxicity and apoptosis in the head and neck cancer (hn-5) cell line

نویسندگان

seyed hadi mousavi medical toxicology research centre, mashhad, university of medical sciences, mashhad, iran.

vahid vazifedan mashhad university of medical sciences

javad sargolzaee mashhad university of medical sciences

shokouhozaman soleymanifard mashhad university of medical sciences

چکیده

malignant tumors of head and neck carcinomas are the sixth most common type of cancer. current systemic therapies for cancer show side effects in normal tissues and short-term efficacy due to drug resistance. consequently, there is much interest in identifying new drugs for cancer treatment. crocin (an active ingredient of saffron) has been shown to have cytotoxic effects on cancer cell lines. chemo radiotherapy is the standard treatment for head and neck cancer. in the present study, the cytotoxic effects, inducing apoptosis and the radiation sensitivity of crocin were evaluated in the head and neck cancer cell line (hn-5). hn-5 cells were cultured in a dmem medium and incubated with different concentrations of crocin (12.5-1000 µg/ml). they were exposed to 2 gy γ-rays. cell viability was quantified by the mtt assay. apoptotic cells were determined using pi staining of dna fragmentation by flowcytometry (sub-g1 peak). crocin decreased cell viability in hn-5 cells in a time and concentration dependent manner. crocin also induced a sub-g1 peak in the flowcytometery histogram of treated cells compared with the control, suggesting that apoptotic cell death is caused by its toxicity. crocin was also shown to sensitize cells to radiation-induced toxicity and apoptosis. the simultaneous use of crocin and radiation therefore increases radiation sensitivity and cell death. thus, after further study crocin can be considered as a potential drug and sensitizer in cancer treatment.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Study of crocin & radiotherapy-induced cytotoxicity and apoptosis in the head and neck cancer (HN-5) cell line

Malignant tumors of head and neck carcinomas are the sixth most common type of cancer. Current systemic therapies for cancer show side effects in normal tissues and short-term efficacy due to drug resistance. Consequently, there is much interest in identifying new drugs for cancer treatment. Crocin (an active ingredient of saffron) has been shown to have cytotoxic effects on cancer cell lines. ...

متن کامل

Study of crocin & radiotherapy-induced cytotoxicity and apoptosis in the head and neck cancer (HN-5) cell line

Malignant tumors of head and neck carcinomas are the sixth most common type of cancer. Current systemic therapies for cancer show side effects in normal tissues and short-term efficacy due to drug resistance. Consequently, there is much interest in identifying new drugs for cancer treatment. Crocin (an active ingredient of saffron) has been shown to have cytotoxic effects on cancer cell lines. ...

متن کامل

Study of Crocin & Radiotherapy-induced Cytotoxicity and Apoptosis in the Head and Neck Cancer (HN-5) Cell Line

Malignant tumors of head and neck carcinomas are the sixth most common type of cancer. Current systemic therapies for cancer show side effects in normal tissues and short-term efficacy due to drug resistance. Consequently, there is much interest in identifying new drugs for cancer treatment. Crocin (an active ingredient of saffron) has been shown to have cytotoxic effects on cancer cell lines. ...

متن کامل

Effect of Clarithromycin on Enhancement of Doxorubicin-induced Cytotoxicity and Apoptosis in A549 Tumor-Cell Line

Background and purpose: Nowadays, multi-drug regimens or combination therapy are new approaches in cancer treatment that combine two or several therapeutic agents. Combination therapy aims at improving the therapeutic efficacy of anticancer drugs while reducing toxicity and preventing drug resistance. The aim of this study was to determine the synergistic effect of combined clarithromycin and d...

متن کامل

P.oleracea induced apoptosis and inhibited the cell growth in oral epithelial cancer cell line

Despite the progress in cancer therapies such as chemotherapy and radiation, its eradication remains as unattainable dream for the patients and doctors. Recently, using supplementary agents such as herbal medicine with fewer side effects seems efficient and attractive. Therefore, the purpose of the present study is to investigate if the ethanolic extract of P.oleracea has cytotoxicity and apopt...

متن کامل

the study of aaag repeat polymorphism in promoter of errg gene and its association with the risk of breast cancer in isfahan region

چکیده: سرطان پستان دومین عامل مرگ مرتبط با سرطان در خانم ها است. از آنجا که سرطان پستان یک تومور وابسته به هورمون است، می تواند توسط وضعیت هورمون های استروئیدی شامل استروژن و پروژسترون تنظیم شود. استروژن نقش مهمی در توسعه و پیشرفت سرطان پستان ایفا می کند و تاثیر خود را روی بیان ژن های هدف از طریق گیرنده های استروژن اعمال می کند. اما گروه دیگری از گیرنده های هسته ای به نام گیرنده های مرتبط به ا...

15 صفحه اول

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of pharmaceutical research

جلد ۱۶، شماره ۱، صفحات ۲۳۰-۲۳۷

کلمات کلیدی
[ ' h n ' , 5 , ' c r o c i n ' , ' c y t o t o x i c i t y ' , ' a p o p t o s i s ' , ' r a d i o t h e r a p y ' ]

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023